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KMID : 0364219960390020215
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Korean Journal of Zoology
1996 Volume.39 No. 2 p.215 ~ p.222
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Substrate Specificity of Mouse Glandular Kallikreins, Epidermal Growth Factor-Binding Protein Type A, B, and c against Mouse Ren 2 Prorenin
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Kim Hwa-Seon
Rhee Hee-Sub
Jeon Byung-Hoon
Kim Won-Sin
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Abstract
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In the previous studies, we have demonstrated that prorenin converting enzyme (PRECE) was identical to the epidermal grouch factor-binding protein (EGF-BP) type B, which was a member of the mouse glandular kallikrein family, To examine whether or not EGF-BP type A and C are involved in the processing of prorenin, we have cloned the CDNAS of the EGF-BP type h and C from a library of male ICR mouse submandibular gland (SMGI. And then CHO cells were transfected with the EGF-BP expression plasmids. and stable cell lines expressing a high level of the EGF-BPS precursor were obtained. The conditioned medium was then treated with trypsin, which has been knotvn to effectively convert the EGF-BP type A and C precursor to the active forms. 수ubsequentlv, the prorenin converting activity of the trypsin-treated or untreated medium was examined. PRECE converted exactly prorenin to renin, but the prorenin converting activities of EGF-BP type A and C were not detected. From these results, it seems that only type B of these EGF-BPs is involved in processing Ren 2 prorenin in mouse SMG.
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KEYWORD
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Submanidbular gland, glandular Kallikrein, Prorenin Converting enzyme, Epidermal Growth Factor-Binding, Orotein, Serine, Protease
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